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Neutrophil elastase antibody

Anti-Neutrophil elastase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03640
Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX03640
Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX03640
Anti-Neutrophil elastase antibody used in Western Blot (WB). GTX03640
Anti-Neutrophil elastase antibody used in Western Blot (WB). GTX03640

Cat. No. GTX03640

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Neutrophil elastase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ELANE(Accession P08246), corresponding to amino acid residues I245-S264.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

elastase, neutrophil expressed , ELA2 , GE , HLE , HNE , NE , PMN-E , SCN1

Cellular Localization

Phagosome

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Neutrophil elastase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03640

GTX03640 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX03640 Neutrophil elastase antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX03640

GTX03640 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX03640 Neutrophil elastase antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX03640

GTX03640 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX03640 Neutrophil elastase antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Neutrophil elastase antibody used in Western Blot (WB). GTX03640

GTX03640 WB Image

WB analysis of HEK293 cell lysate using GTX03640 Neutrophil elastase antibody. The lane on the left was treated with blocking peptide.

Anti-Neutrophil elastase antibody used in Western Blot (WB). GTX03640

GTX03640 WB Image

WB analysis of HEK293 cell lysate using GTX03640 Neutrophil elastase antibody. The lane on the left was treated with blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399