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Neutrophil elastase antibody

Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX66150
Anti-Neutrophil elastase antibody used in Western Blot (WB). GTX66150
Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX66150

Cat. No. GTX66150

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399)
See all Neutrophil elastase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 30-267 of human ELANE (NP_001963.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

elastase, neutrophil expressed , ELA2 , GE , HLE , HNE , NE , PMN-E , SCN1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX66150

GTX66150 IHC-P Image

IHC-P analysis of mouse bone marrow tissue using GTX66150 Neutrophil elastase antibody.
Blue : DAPI
Dilution : 1:100

Anti-Neutrophil elastase antibody used in Western Blot (WB). GTX66150

GTX66150 WB Image

WB analysis of rat spleen tissue lysate using GTX66150 Neutrophil elastase antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:500
Loading : 25μg per lane

Anti-Neutrophil elastase antibody used in IHC (Paraffin sections) (IHC-P). GTX66150

GTX66150 IHC-P Image

IHC-P analysis of rat bone marrow tissue using GTX66150 Neutrophil elastase antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Perico L et al. Sci Rep 2023; 13 (1) : 11392 SARS-CoV-2 spike protein induces lung endothelial cell dysfunction and thrombo-inflammation depending on the C3a/C3a receptor signalling.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399