GeneTex
United States (US)

Nicastrin antibody

GTX115930_40856_IFA_18121410_841.jpg
GTX115930_40856_WB_18121410_632.jpg

Cat No. GTX115930

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nicastrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APH2 antibody, KIAA0253 antibody, NCSTN antibody, nicastrin antibody, anterior pharynx-defective 2 antibody, ATAG1874 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein (Potential) , Melanosome

Background

This gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115930_40856_IFA_18121410_841.jpg

GTX115930 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Nicastrin(GTX115930) antibody at 1:500 dilution.

GTX115930_40856_WB_18121410_632.jpg

GTX115930 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HeLa
C: HepG2
5% SDS PAGE
GTX115930 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf