GeneTex
United States (US)

Nicastrin antibody

Anti-Nicastrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31751
Anti-Nicastrin antibody used in Western Blot (WB). GTX31751
Anti-Nicastrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31751

Cat No. GTX31751

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918) , Mouse Brain Tissue Lysate(GTX27934)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nicastrin antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human Nicastrin.The immunogen is located within amino acids 630 - 680 of Nicastrin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nicastrin,Atag1874,Ncstn

Background

This gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Nicastrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31751

GTX31751 IHC-P Image

IHC-P analysis of human brain tissue using GTX31751 Nicastrin antibody.
Working concentration : 5 μg/ml

Anti-Nicastrin antibody used in Western Blot (WB). GTX31751

GTX31751 WB Image

WB analysis of mouse brain tissue lysate using GTX31751 Nicastrin antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-Nicastrin antibody used in IHC (Paraffin sections) (IHC-P). GTX31751

GTX31751 IHC-P Image

IHC-P analysis of human brain tissue using GTX31751 Nicastrin antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329