GeneTex
United States (US)

Nitrotyrosine antibody [39B6]

Cat No. GTX30730

Host Mouse
Clonality Monoclonal
Clone Name 39B6
Isotype IgG2a
Application WB, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat, Dog
APPLICATION

Application Note

For WB: Use at a concentration of 0.2-2ug/ml For IHC: Use at a concentration of 1-5ug/ml. For IP: Use 1-2ug per 100-500ug total protein. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Rat brain tissue extract or nitrated BSA

Specificity/Sensitivity

This antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples.
PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol with 0.02% sodium azide as preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

3-(4-hydroxy-3-nitrophenylacetamido) propionic acid conjugated to BSA

Purification

Protein G affinity purified.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

3 Nitrotyrosine, 3Nitrotyrosine

Background

Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.
REFERENCE
Package List Price ($)
$ 239