For WB: Use at a concentration of 0.2-2ug/ml For IHC: Use at a concentration of 1-5ug/ml. For IP: Use 1-2ug per 100-500ug total protein. Optimal dilutions/concentrations should be determined by the end user.
Rat brain tissue extract or nitrated BSA
This antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples.
PBS, 50% glycerol with 0.02% sodium azide as preservative
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
3-(4-hydroxy-3-nitrophenylacetamido) propionic acid conjugated to BSA
Protein G affinity purified.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
3 Nitrotyrosine, 3Nitrotyrosine
Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.