GeneTex
United States (US)

Nkx2.2 antibody [NX2/1422R]

Anti-Nkx2.2 antibody [NX2/1422R] used in IHC (Paraffin sections) (IHC-P). GTX34913

Cat No. GTX34913

Host Rabbit
Clonality Monoclonal
Clone Name NX2/1422R
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Specificity/Sensitivity

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human NKX2.2 protein fragment (exact sequence is proprietary)

Purification

Ab purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nk2 Homeobox 2,Nkx2.2,Nkx2B,Nkx2-2

Background

The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Nkx2.2 antibody [NX2/1422R] used in IHC (Paraffin sections) (IHC-P). GTX34913

GTX34913 IHC-P Image

Formalin-fixed, paraffin-embedded human Ewing’s Sarcoma stained with NKX2.2 Recombinant Rabbit Monoclonal Antibody (NX2/1422R).

Package List Price ($)
$ 379