GeneTex
United States (US)

Nkx2.2 antibody

Anti-Nkx2.2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31470
Anti-Nkx2.2 antibody used in Western Blot (WB). GTX31470
Anti-Nkx2.2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31470

Cat No. GTX31470

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Rat Kidney Tissue Lysate(GTX27944) , Human Kidney Tissue Slide

Specificity/Sensitivity

NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2.The immunogen is located within amino acids 90 - 140 of NKX2-2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nk2 Homeobox 2,Nkx2.2,Nkx2B,Nkx2-2

Background

The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Nkx2.2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31470

GTX31470 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31470 Nkx2.2 antibody.
Working concentration : 20 μg/ml

Anti-Nkx2.2 antibody used in Western Blot (WB). GTX31470

GTX31470 WB Image

WB analysis of rat kidney tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31470 Nkx2.2 antibody.
Working concentration : 1 μg/ml

Anti-Nkx2.2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31470

GTX31470 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31470 Nkx2.2 antibody.
Working concentration : 2.5 μg/ml

Package List Price ($)
$ 329