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Nkx2.4 antibody

Anti-Nkx2.4 antibody used in Western Blot (WB). GTX32058
Anti-Nkx2.4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32058
Anti-Nkx2.4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32058

Cat No. GTX32058

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A-20 Cell Slide(GTX25541) , A20 Cell Lysate(GTX27180)

Specificity/Sensitivity

NKX2-4 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-4 are known to exist; this antibody will detect both isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NKX2-4 antibody was raised against an 18 amino acid synthetic peptide near the center of human NKX2-4.The immunogen is located within amino acids 220 - 270 of NKX2-4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NK2 homeobox 4 , NKX2.4 , NKX2D

Cellular Localization

Nucleus

Database

DATA IMAGES

Anti-Nkx2.4 antibody used in Western Blot (WB). GTX32058

GTX32058 WB Image

WB analysis of A-20 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32058 Nkx2.4 antibody.
Working concentration : 1 μg/ml

Anti-Nkx2.4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32058

GTX32058 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX32058 Nkx2.4 antibody.
Working concentration : 20 μg/ml

Anti-Nkx2.4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32058

GTX32058 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX32058 Nkx2.4 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

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Package List Price ($)
$ 329