*Optimal dilutions/concentrations should be determined by the researcher.
Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88402-PEP).
This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Mouse, Rat, Bovine, Dog(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nk2 Homeobox 5,Chng5,Csx,Csx1,Hlhs2,Nkx2.5,Nkx2E,Nkx4-1,Vsd3,Nkx2-5
This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
GTX88402 IHC-P Image
IHC-P analysis of human heart using GTX88402 Nkx2.5 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml
GTX88402 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX88402 Nkx2.5 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5µg/ml
GTX88402 WB Image
WB analysis of human heart lysate using GTX88402 Nkx2.5 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer