GeneTex
United States (US)

Nkx2.5 antibody, Internal

GTX88402_971_IHC-P_18122102_408.jpg
GTX88402_128_ICC-IF_18122102_708.jpg
GTX88402_2352_WB_18122102_944.jpg

Cat No. GTX88402

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
ICC/IF 5μg/ml
IHC-P 3.75μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88402-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nk2 Homeobox 5,Chng5,Csx,Csx1,Hlhs2,Nkx2.5,Nkx2E,Nkx4-1,Vsd3,Nkx2-5

Background

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX88402_971_IHC-P_18122102_408.jpg

GTX88402 IHC-P Image

IHC-P analysis of human heart using GTX88402 Nkx2.5 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88402_128_ICC-IF_18122102_708.jpg

GTX88402 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX88402 Nkx2.5 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5µg/ml

GTX88402_2352_WB_18122102_944.jpg

GTX88402 WB Image

WB analysis of human heart lysate using GTX88402 Nkx2.5 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329