APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 5μg/ml |
| 3.75μg/ml |
|
Note :
ICC/IF
Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88402-PEP).
Product Note
This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NK2 homeobox 5 , CHNG5 , CSX , CSX1 , HLHS2 , NKX2.5 , NKX2E , NKX4-1 , VSD3
Cellular Localization
Nucleus
Background
This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
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GTX88402 IHC-P Image
IHC-P analysis of human heart using GTX88402 Nkx2.5 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX88402 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX88402 Nkx2.5 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5μg/ml
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GTX88402 WB Image
WB analysis of human heart lysate using GTX88402 Nkx2.5 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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