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Nkx2.5 antibody

Comparison Orthogonal
Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711
Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711
Anti-Nkx2.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105711
Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX105711
Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX105711
Anti-Nkx2.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105711
Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711
Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711

Cat. No. GTX105711

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Nkx2.5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nkx2.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950999

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NK2 homeobox 5 , CHNG5 , CSX , CSX1 , HLHS2 , NKX2.5 , NKX2E , NKX4-1 , VSD3

Cellular Localization

Nucleus

Background

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711

GTX105711 WB Image

Comparison

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711

GTX105711 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nkx2.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105711

GTX105711 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(GTX105711) antibody at 1:200 dilution.

Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX105711

GTX105711 IHC-P Image

Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat heart. Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX105711

GTX105711 IHC-P Image

Nkx2.5 antibody detects Nkx2.5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nkx2.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105711

GTX105711 ICC/IF Image

Nkx2.5 antibody detects Nkx2.5 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711

GTX105711 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nkx2.5 antibody used in Western Blot (WB). GTX105711

GTX105711 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Gasi?nien? M et al. Int J Mol Sci 2020; 21 (22) The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.
Marunouchi T et al. Biomed Pharmacother 2018; 108 : 883-892 Effects of cardiosphere-derived cell transplantation on cardiac mitochondrial oxygen consumption after myocardial infarction in rats.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169