GeneTex
United States (US)

Nkx2.5 antibody

GTX105711_42662_20171201_IHC-P_R_19011506_223.jpg
GTX105711_39953_IFA_mESC_19011506_308.jpg
GTX105711_41850_20161020_WB_19011506_982.jpg
GTX105711_42662_20161110_WB_M_19011506_617.jpg

Cat No. GTX105711

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A549 , mouse heart

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.03mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nkx2.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nk2 Homeobox 5,Chng5,Csx,Csx1,Hlhs2,Nkx2.5,Nkx2E,Nkx4-1,Vsd3,Nkx2-5

Cellular Localization

Nucleus

Background

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX105711_42662_20171201_IHC-P_R_19011506_223.jpg

GTX105711 IHC-P Image

Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105711_39953_IFA_mESC_19011506_308.jpg

GTX105711 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(GTX105711) antibody at 1:200 dilution.

GTX105711_41850_20161020_WB_19011506_982.jpg

GTX105711 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105711_42662_20161110_WB_M_19011506_617.jpg

GTX105711 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169