*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A549 , mouse heart
Bovine, Dog(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
2.03mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Nkx2.5. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nk2 Homeobox 5,Chng5,Csx,Csx1,Hlhs2,Nkx2.5,Nkx2E,Nkx4-1,Vsd3,Nkx2-5
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]
GTX105711 IHC-P Image
Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Nkx2.5 stained by Nkx2.5 antibody (GTX105711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX105711 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(GTX105711) antibody at 1:200 dilution.
GTX105711 WB Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX105711 WB Image
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX105711) diluted at 1:1000.