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Nkx2.5 antibody

Comparison Orthogonal
Anti-Nkx2.5 antibody used in Western Blot (WB). GTX133155
Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155
Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155

Cat No. GTX133155

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Nkx2.5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Human, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse Nkx2.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NK2 homeobox 5 , Csx , Nkx-2.5 , Nkx2.5 , tinman

Cellular Localization

Nucleus

Background

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4.

Database

Research Area

DATA IMAGES

Anti-Nkx2.5 antibody used in Western Blot (WB). GTX133155

GTX133155 WB Image

Comparison Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Nkx2.5 antibody (GTX133155) diluted at 1:500 and competitor's antibody (sc-14033) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155

GTX133155 IHC-P Image

Nkx2.5 antibody detects Nkx2.5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: Nkx2.5 stained by Nkx2.5 antibody (GTX133155) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155

GTX133155 IHC-P Image

Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Nkx2.5 stained by Nkx2.5 antibody (GTX133155) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Koch L et al. Biofabrication 2018; (Epub) Laser bioprinting of human induced pluripotent stem cells - the effect of printing and biomaterials on cell survival, pluripotency, and differentiation.
Application : ICC/IF
Lao S et al. Mol Med Rep 2017; 16 (1) : 507-514 A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
Application : WB
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169