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Nkx2.5 antibody

Comparison Orthogonal
Anti-Nkx2.5 antibody used in Western Blot (WB). GTX133155
Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155
Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155
Anti-Nkx2.5 antibody used in Western Blot (WB). GTX133155

Cat No. GTX133155

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Nkx2.5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Human, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse Nkx2.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886848

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NK2 homeobox 5 , Csx , Nkx-2.5 , Nkx2.5 , tinman

Cellular Localization

Nucleus

Background

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4.

Database

Research Area

DATA IMAGES

Anti-Nkx2.5 antibody used in Western Blot (WB). GTX133155

GTX133155 WB Image

Comparison Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Nkx2.5 antibody (GTX133155) diluted at 1:500 and competitor's antibody (sc-14033) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155

GTX133155 IHC-P Image

Nkx2.5 antibody detects Nkx2.5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: Nkx2.5 stained by Nkx2.5 antibody (GTX133155) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nkx2.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX133155

GTX133155 IHC-P Image

Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Nkx2.5 stained by Nkx2.5 antibody (GTX133155) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nkx2.5 antibody used in Western Blot (WB). GTX133155

GTX133155 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX133155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Koch L et al. Biofabrication 2018; (Epub) Laser bioprinting of human induced pluripotent stem cells - the effect of printing and biomaterials on cell survival, pluripotency, and differentiation.
Application : ICC/IF
Lao S et al. Mol Med Rep 2017; 16 (1) : 507-514 A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
Application : WB
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169