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Nkx2.6 antibody

Anti-Nkx2.6 antibody used in Western Blot (WB). GTX32059
Anti-Nkx2.6 antibody used in IHC (Paraffin sections) (IHC-P). GTX32059
Anti-Nkx2.6 antibody used in IHC (Paraffin sections) (IHC-P). GTX32059

Cat No. GTX32059

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Mouse Heart Tissue Lysate(GTX27932) , Human Spleen Tissue Slide

Specificity/Sensitivity

NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this order of antibodies will detect both isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NKX2-6 antibody was raised against a 15 amino acid synthetic peptide near the center of human NKX2-6.The immunogen is located within amino acids 170 - 220 of NKX2-6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NK2 homeobox 6 , CSX2 , CTHM , NKX2F , NKX4-2

Cellular Localization

Nucleus

Background

This gene encodes a homeobox-containing protein that belongs to the NK-2 homeobox family. This protein is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are associated with persistent truncus arteriosus.[provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Nkx2.6 antibody used in Western Blot (WB). GTX32059

GTX32059 WB Image

WB analysis of mouse heart tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX32059 Nkx2.6 antibody.
Working concentration : 1 μg/ml

Anti-Nkx2.6 antibody used in IHC (Paraffin sections) (IHC-P). GTX32059

GTX32059 IHC-P Image

IHC-P analysis of human spleen tissue using GTX32059 Nkx2.6 antibody.
Working concentration : 5 μg/ml

Anti-Nkx2.6 antibody used in IHC (Paraffin sections) (IHC-P). GTX32059

GTX32059 IHC-P Image

IHC-P analysis of human spleen tissue using GTX32059 Nkx2.6 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 329