APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Heart Tissue Lysate(GTX27932) , Human Spleen Tissue Slide
Product Note
NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this order of antibodies will detect both isoforms.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
NKX2-6 antibody was raised against a 15 amino acid synthetic peptide near the center of human NKX2-6.The immunogen is located within amino acids 170 - 220 of NKX2-6.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NK2 homeobox 6 , CSX2 , CTHM , NKX2F , NKX4-2
Cellular Localization
Nucleus
Background
This gene encodes a homeobox-containing protein that belongs to the NK-2 homeobox family. This protein is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are associated with persistent truncus arteriosus.[provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
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GTX32059 IHC-P Image
IHC-P analysis of human spleen tissue using GTX32059 Nkx2.6 antibody.
Working concentration : 20 μg/ml
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GTX32059 IHC-P Image
IHC-P analysis of human spleen tissue using GTX32059 Nkx2.6 antibody.
Working concentration : 5 μg/ml
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GTX32059 WB Image
WB analysis of mouse heart tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX32059 Nkx2.6 antibody.
Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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