Nodal antibody, Internal

Cat. No. GTX88954

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	ELISA, Sandwich ELISA
Reactivity	Human
Package	100 μg ($399)

See all Nodal products

Datasheet
Document

View product citations for antibody GTX88954 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ELISA	Assay dependent
Sandwich ELISA	Assay dependent

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88954-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence ECPNPVGEEFH, from the internal region of the protein sequence according to NP_060525.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nodal growth differentiation factor , HTX5

Cellular Localization

Secreted

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016]

Database

Gene ID: 4838 NODAL
UniProt: Q96S42 NODAL

Research Area

DATA IMAGES

GTX88954 ELISA Image

ELISA analysis of Nodal protein using GTX88954 Nodal antibody, Internal.
Capture : GTX106644 (5μg/ml)
Detection : GTX88954 (1.5μg/ml)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty