GeneTex
United States (US)

Nodal antibody

GTX106644_39959_IHC_19011506_586.jpg
GTX106644_39959_20151123_IHC-P_R_19011506_414.jpg
GTX106644_39959_20151119_WB_M_brain_19011506_361.jpg
NODAL_ELISA-PAIR_19011506_144.jpg
GTX106644_39959_WB_hESC_19011506_106.jpg

Cat No. GTX106644

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Human ESC

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC138230 antibody, NODAL antibody, nodal homolog antibody, nodal homolog (mouse) antibody

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106644_39959_IHC_19011506_586.jpg

GTX106644 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Nodal(GTX106644) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106644_39959_20151123_IHC-P_R_19011506_414.jpg

GTX106644 IHC-P Image

Nodal antibody detects NODAL protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Nodal antibody (GTX106644) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106644_39959_20151119_WB_M_brain_19011506_361.jpg

GTX106644 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Nodal antibody (GTX106644) diluted at 1:500.

NODAL_ELISA-PAIR_19011506_144.jpg

GTX106644 ELISA Image

ELISA detection of NODAL using GTX106644 for capture at a concentration of 5 µg/mL and GTX88954 for detection at a concentration of 1.5 µg/mL.

GTX106644_39959_WB_hESC_19011506_106.jpg

GTX106644 WB Image

Sample (20 ug of whole cell lysate)
A: Human ESC
10% SDS PAGE
GTX106644 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169