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Nodal antibody

Anti-Nodal antibody used in IHC (Paraffin sections) (IHC-P). GTX106644
Anti-Nodal antibody used in Western Blot (WB). GTX106644
Anti-Nodal antibody used in Western Blot (WB). GTX106644
Anti-Nodal antibody used in IHC (Paraffin sections) (IHC-P). GTX106644
Anti-Nodal antibody used in ELISA (ELISA). GTX106644

Cat. No. GTX106644

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Nodal products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX106644 , Detection: GTX88954
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Human ESC , mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037537

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nodal growth differentiation factor , HTX5

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Nodal antibody used in IHC (Paraffin sections) (IHC-P). GTX106644

GTX106644 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Nodal(GTX106644) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nodal antibody used in Western Blot (WB). GTX106644

GTX106644 WB Image

Sample (20 ug of whole cell lysate)
A: Human ESC
10% SDS PAGE
GTX106644 diluted at 1:1000

Anti-Nodal antibody used in Western Blot (WB). GTX106644

GTX106644 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Nodal antibody (GTX106644) diluted at 1:500.

Anti-Nodal antibody used in IHC (Paraffin sections) (IHC-P). GTX106644

GTX106644 IHC-P Image

Nodal antibody detects NODAL protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Nodal antibody (GTX106644) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nodal antibody used in ELISA (ELISA). GTX106644

GTX106644 ELISA Image

ELISA detection of NODAL using GTX106644 for capture at a concentration of 5 μg/mL and GTX88954 for detection at a concentration of 1.5 μg/mL.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169