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Noelin antibody [C3], C-term

Anti-Noelin antibody [C3], C-term used in Western Blot (WB). GTX108528
Anti-Noelin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108528
Anti-Noelin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108528
Anti-Noelin antibody [C3], C-term used in Western Blot (WB). GTX108528

Cat. No. GTX108528

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Noelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Mouse brain , HepG2

Predict Reactivity

Rat, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Noelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951062

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

olfactomedin 1 , AMY , NOE1 , NOELIN1 , OlfA

Cellular Localization

Endoplasmic reticulum lumen

Background

This gene product shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of the encoded protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Noelin antibody [C3], C-term used in Western Blot (WB). GTX108528

GTX108528 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108528 diluted at 1:1000

Anti-Noelin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108528

GTX108528 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Noelin(GTX108528) antibody at 1:500 dilution.

Anti-Noelin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108528

GTX108528 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Noelin(GTX108528) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Noelin antibody [C3], C-term used in Western Blot (WB). GTX108528

GTX108528 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX108528 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169