GeneTex
United States (US)

Noelin antibody [C3], C-term

GTX108528_39855_IHC_19011507_506.jpg
GTX108528_39855_WB_M_brain_19011507_196.jpg
GTX108528_WB_19011507_579.jpg
GTX108528_39855_IFA_19011507_479.jpg

Cat No. GTX108528

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HepG2 , mouse brain

Predict Reactivity

Rat, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Noelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Olfactomedin 1,Amy,Noe1,Noelin1,Olfa,Olfm1

Cellular Localization

Endoplasmic reticulum lumen

Background

This gene product shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of the encoded protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108528_39855_IHC_19011507_506.jpg

GTX108528 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Noelin(GTX108528) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108528_39855_WB_M_brain_19011507_196.jpg

GTX108528 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108528 diluted at 1:1000

GTX108528_WB_19011507_579.jpg

GTX108528 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX108528 diluted at 1:500

GTX108528_39855_IFA_19011507_479.jpg

GTX108528 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Noelin(GTX108528) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169