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Noggin antibody [4C12]

Overexpression
Anti-Noggin antibody [4C12] used in IHC (Paraffin sections) (IHC-P). GTX84023
Anti-Noggin antibody [4C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84023
Anti-Noggin antibody [4C12] used in Western Blot (WB). GTX84023
Anti-Noggin antibody [4C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84023
Anti-Noggin antibody [4C12] used in Western Blot (WB). GTX84023
Anti-Noggin antibody [4C12] used in Western Blot (WB). GTX84023

Cat. No. GTX84023

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4C12

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($519)
See all Noggin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

noggin , SYM1 , SYNS1 , SYNS1A

Cellular Localization

Secreted

Background

Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite.

Database

Research Area

DATA IMAGES

Anti-Noggin antibody [4C12] used in IHC (Paraffin sections) (IHC-P). GTX84023

GTX84023 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84023 Noggin antibody [4C12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Noggin antibody [4C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84023

GTX84023 ICC/IF Image

ICC/IF analysis of A549 cells using GTX84023 Noggin antibody [4C12].

Anti-Noggin antibody [4C12] used in Western Blot (WB). GTX84023

GTX84023 WB Image

WB analysis of recombinant human Noggi using GTX84023 Noggin antibody [4C12].
Dilution : 1:2000

Anti-Noggin antibody [4C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84023

GTX84023 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Noggin plasmid using GTX84023 Noggin antibody [4C12].

Anti-Noggin antibody [4C12] used in Western Blot (WB). GTX84023

GTX84023 WB Image

WB analysis of various cell lines using GTX84023 Noggin antibody [4C12].
Loading : 35 ug per lane

Anti-Noggin antibody [4C12] used in Western Blot (WB). GTX84023

GTX84023 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Noggin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84023 Noggin antibody [4C12].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519