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Nogo A antibody

Anti-Nogo A antibody used in Western Blot (WB). GTX48703
Anti-Nogo A antibody used in Immunohistochemistry (IHC). GTX48703

Cat No. GTX48703

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC

Reactivity

Human, Mouse, Rat
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1.0 μg/mL
ELISA 1:10000 - 1:40000
IHC 2.5 μg/mL
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Specificity/Sensitivity

At least five isoforms of Nogo are known to exist; this antibody is specific for Nogo A and Nogo E.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Nogo A protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

reticulon 4 , ASY , NI220/250 , NOGO , NSP , NSP-CL , Nbla00271 , Nbla10545 , RTN-X , RTN4-A , RTN4-B1 , RTN4-B2 , RTN4-C

Cellular Localization

Cell membrane; Multi-pass membrane protein,Cell membrane,Cell junction

Background

NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.

Database

Research Area

DATA IMAGES

Anti-Nogo A antibody used in Western Blot (WB). GTX48703

GTX48703 WB Image

Western blot demonstrating detection of NogoA in human brain tissue lysate. Primary antibody was used at concentration of 0.5 μg/mL (A) and 1.0 μg/mL (B).

Anti-Nogo A antibody used in Immunohistochemistry (IHC). GTX48703

GTX48703 IHC Image

Immunohistochemistry showing detection of NogoA in mouse brain tissue. Primary antibody was used at 2.5 μg/ml.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf