*Optimal dilutions/concentrations should be determined by the researcher.
|0.5 - 1.0 μg/mL
|1:10000 - 1:40000
Not tested in other applications.
At least five isoforms of Nogo are known to exist; this antibody is specific for Nogo A and Nogo E.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Nogo A protein.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
reticulon 4 , ASY , NI220/250 , NOGO , NSP , NSP-CL , Nbla00271 , Nbla10545 , RTN-X , RTN4-A , RTN4-B1 , RTN4-B2 , RTN4-C
Cell membrane; Multi-pass membrane protein,Cell membrane,Cell junction
NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.
GTX48703 WB Image
Western blot demonstrating detection of NogoA in human brain tissue lysate. Primary antibody was used at concentration of 0.5 μg/mL (A) and 1.0 μg/mL (B).
GTX48703 IHC Image
Immunohistochemistry showing detection of NogoA in mouse brain tissue. Primary antibody was used at 2.5 μg/ml.