APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| Assay dependent |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Lysate(GTX27918) , Mouse Brain Tissue Lysate(GTX27934)
Product Note
At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA and NogoE.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
NogoA antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA.The immunogen is located within amino acids 200 - 250 of NogoA.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_10724078
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
reticulon 4 , ASY , NI220/250 , NOGO , NSP , NSP-CL , Nbla00271 , Nbla10545 , RTN-X , RTN4-A , RTN4-B1 , RTN4-B2 , RTN4-C
Cellular Localization
Cell membrane; Multi-pass membrane protein,Cell membrane,Cell junction
Background
NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients. Despite its predicted molecular weight, NogoA typically migrates at 180kDa in an SDS-PAGE. At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA and NogoE.
Database
Research Area
DATA IMAGES
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GTX85447 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85447 Nogo A antibody.
Working concentration : 20 μg/ml
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GTX85447 WB Image
WB analysis of human brain tissue lysate using GTX85447 Nogo A antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX85447 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85447 Nogo A antibody.
Working concentration : 5 μg/ml
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GTX85447 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85447 Nogo A antibody.
Working concentration : 20 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin
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REFERENCE
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