Immunofluorescence analysis of paraformaldehyde-fixed A431, using Nop25(GTX120303) antibody at 1:500 dilution.

Nop25 antibody detects Nop25 protein at nucleous on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Nop25 antibody (GTX120303) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX120303 diluted at 1:1000

Nop25 antibody detects NOL12 protein at nucleous on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Nop25 antibody (GTX120303) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX120303 diluted at 1:1000

Nop25 antibody detects Nop25 protein at nucleous on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Nop25 antibody (GTX120303) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min