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Nop5p + Nop58p antibody [34B12]

Anti-Nop5p + Nop58p antibody [34B12] used in Western Blot (WB). GTX24639

Cat. No. GTX24639

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 34B12

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Yeast
Package
250 μl ($399)
See all Nop5p products

APPLICATION

Application Note

IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). IP: Use 2-4 μl for the amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (ECL). Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Does not immunoprecipitate as well as another Nop5p monoclonal antibody, GTX24640. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

10mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Yeast

Immunogen

This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

Purification

Tissue culture supernatant
Sterile filtered.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus, nucleolus

Database

Research Area

DATA IMAGES

Anti-Nop5p + Nop58p antibody [34B12] used in Western Blot (WB). GTX24639

GTX24639 WB Image

Antibody recognizes a single 57kDa band in blots of whole yeast protein extracts.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399