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Norrin antibody

Anti-Norrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85292
Anti-Norrin antibody used in Western Blot (WB). GTX85292

Cat. No. GTX85292

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Norrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Norrin antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human Norrin.The immunogen is located within the first 50 amino acids of Norrin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

norrin cystine knot growth factor NDP , EVR2 , FEVR , ND

Cellular Localization

Secreted

Background

Norrie disease is an X-linked genetic disorder characterized by progressive atrophy of the eyes, mental disturbances and deafness. The gene responsible for this disease was initially identified through positional cloning. Norrin, the gene product, encodes a small secreted, cysteine-rich protein that is thought to act as a ligand for the Wnt-receptor/b-catenin signal pathway despite having sequence homology with the Wnt family of proteins. Mice lacking this gene have abnormal blood vessel growth in the vitreous and a disorganized retina; transgenic ectopic expression of Norrin restores normal retinal vasculature. Recent evidence shows that Norrin can attenuate tPA and uPA-mediated death of transformed rat retinal ganglion cells (RGC-5) by activating the Wnt/b-catenin pathway and regulating the phosphorylation of LRP-1, a cell surface receptor for tPA and uPA, suggesting the Norrin may function in vivo by regulating kinases which may alter the phosphorylation of LRP-1.

Database

Research Area

DATA IMAGES

Anti-Norrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85292

GTX85292 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX85292 Norrin antibody.
Working concentration : 5 μg/ml

Anti-Norrin antibody used in Western Blot (WB). GTX85292

GTX85292 WB Image

WB analysis of Jurkat cell lysate using GTX85292 Norrin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399