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Nsp1p antibody [32D6]

Anti-Nsp1p antibody [32D6] used in Western Blot (WB). GTX24641

Cat. No. GTX24641

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 32D6

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Yeast
Package
250 μl ($399)
See all Nsp1p products

APPLICATION

Application Note

IF: Use at a dilution of 1/1000 - 1/1000 (yeast cells). WB: Use at a dilution of 1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, use at a dilution of 1/1000 - 1/5000. Detects a band of approximately 108 kDa and 91 kDa. Optimal dilutions/concentrations should be determined by the end user.

Product Note

This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for Nsp1p, strain RS453, which expresses a shortened isoform of Nsp1p, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype Nsp1p is 86.5 kDa, but Nsp1p has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype Nsp1p was 108 kDa, whereas the short isoform of Nsp1p in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein Nsp1p.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

10mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

Purification

Tissue culture supernatant
Sterile filtered.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Nsp1p antibody [32D6] used in Western Blot (WB). GTX24641

GTX24641 WB Image

Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for GTX24641 is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kDa.

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399