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NuMA antibody [A73-B/D12]

Anti-NuMA antibody [A73-B/D12] used in IHC (Paraffin sections) (IHC-P). GTX34917

Cat No. GTX34917

Host Mouse
Clonality Monoclonal
Clone Name A73-B/D12
Isotype IgM
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

238 kDa. ( Note )

Specificity/Sensitivity

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Colon carcinoma 174T cells

Purification

Ab purified from Bioreactor Concentrate

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Mitotic Apparatus Protein 1,Nmp-22,Numa,Numa1

Background

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES
Anti-NuMA antibody [A73-B/D12] used in IHC (Paraffin sections) (IHC-P). GTX34917

GTX34917 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with NuMA Monoclonal Antibody (A73-B/D12)

Package List Price ($)
$ 329