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NuMA antibody [GT1510]

KO/KD
Anti-NuMA antibody [GT1510] used in Western Blot (WB). GTX629072
Anti-NuMA antibody [GT1510] used in Western Blot (WB). GTX629072
Anti-NuMA antibody [GT1510] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629072

Cat. No. GTX629072

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1510

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NuMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

238 kDa. ( Note )

Positive Control

A375 , 293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NuMA. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888090

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear mitotic apparatus protein 1 , NMP-22 , NUMA

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-NuMA antibody [GT1510] used in Western Blot (WB). GTX629072

GTX629072 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [GT1510] (GTX629072) diluted at 1:500.

Anti-NuMA antibody [GT1510] used in Western Blot (WB). GTX629072

GTX629072 WB Image

NuMA antibody [GT1510] detects NUMA1 protein by Western blot analysis.
A. 30 μg A375 whole cell lysate/extract
5 % SDS-PAGE
NuMA antibody [GT1510] (GTX629072) dilution: 1:1000

Anti-NuMA antibody [GT1510] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629072

GTX629072 ICC/IF Image

NuMA antibody [GT1510] detects NuMA protein at centrosome by immunofluorescent analysis. Mitotic cells are highlighted in yellow box.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NuMA protein stained by NuMA antibody [GT1510] (GTX629072) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations