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NuMA antibody [GT3611]

KO/KD
Anti-NuMA antibody [GT3611] used in Western Blot (WB). GTX629397
Anti-NuMA antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629397
Anti-NuMA antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629397
Anti-NuMA antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629397
Anti-NuMA antibody [GT3611] used in Western Blot (WB). GTX629397
Anti-NuMA antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX629397
Anti-NuMA antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX629397

Cat. No. GTX629397

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3611

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all NuMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

238 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NuMA. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888103

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear mitotic apparatus protein 1 , NMP-22 , NUMA

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-NuMA antibody [GT3611] used in Western Blot (WB). GTX629397

GTX629397 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [GT3611] (GTX629397) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NuMA antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629397

GTX629397 ICC/IF Image

NuMA antibody [GT3611] detects NuMA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NuMA stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.

Anti-NuMA antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629397

GTX629397 ICC/IF Image

NuMA antibody [GT3611] detects NuMA protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NuMA protein stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NuMA antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629397

GTX629397 ICC/IF Image

NuMA antibody [GT3611] detects NuMA protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NuMA protein stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NuMA antibody [GT3611] used in Western Blot (WB). GTX629397

GTX629397 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [GT3611] (GTX629397) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NuMA antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX629397

GTX629397 IHC-P Image

NuMA antibody [GT3611] detects NuMA protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NuMA stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NuMA antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX629397

GTX629397 IHC-P Image

NuMA antibody [GT3611] detects NuMA protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NuMA stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Mitsiadis TA et al. Adv Exp Med Biol 2021; 1331 : 65-75 The Versatile Roles of Nerve Growth Factor in Neuronal Attraction, Odontoblast Differentiation, and Mineral Deposition in Human Teeth.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169