Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [GT3611] (GTX629397) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

NuMA antibody [GT3611] detects NuMA protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NuMA stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NuMA antibody [GT3611] detects NuMA protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NuMA stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [GT3611] (GTX629397) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

NuMA antibody [GT3611] detects NuMA protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NuMA protein stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:500.
Blue: Hoechst 33342 staining.

NuMA antibody [GT3611] detects NuMA protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NuMA protein stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:500.
Blue: Hoechst 33342 staining.

NuMA antibody [GT3611] detects NuMA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NuMA stained by NuMA antibody [GT3611] (GTX629397) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.