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NuMA antibody [SPM300]

Anti-NuMA antibody [SPM300] used in IHC (Paraffin sections) (IHC-P). GTX34918

Cat. No. GTX34918

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM300

Isotype

 IgM

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all NuMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

238 kDa. ( Note )

Product Note

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Colon carcinoma 174T cells

Purification

PEG precipitation

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NMP22 , NUMA , NUMA1 , nuclear mitotic apparatus protein 1 , NuMA

Cellular Localization

Nucleus,Nucleoplasm,Nucleus matrix,Chromosome,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle pole,Cell cortex,Cell membrane,Lateral cell membrane,Cytosol

Background

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES

Anti-NuMA antibody [SPM300] used in IHC (Paraffin sections) (IHC-P). GTX34918

GTX34918 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34918 NuMA antibody [SPM300].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529