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NuMA antibody

Anti-NuMA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64368
Anti-NuMA antibody used in IHC (Paraffin sections) (IHC-P). GTX64368
Anti-NuMA antibody used in Western Blot (WB). GTX64368
Anti-NuMA antibody used in IHC (Paraffin sections) (IHC-P). GTX64368
Anti-NuMA antibody used in IHC (Paraffin sections) (IHC-P). GTX64368

Cat. No. GTX64368

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NuMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:1000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

238 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human NUMA1 (NP_006176.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear mitotic apparatus protein 1 , NMP-22 , NUMA

Cellular Localization

Nucleus,nucleoplasm,Cytoplasm, cytoskeleton,Cell membrane

Background

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES

Anti-NuMA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64368

GTX64368 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64368 NuMA antibody.
Blue : DAPI
Dilution : 1:100

Anti-NuMA antibody used in IHC (Paraffin sections) (IHC-P). GTX64368

GTX64368 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX64368 NuMA antibody.
Dilution : 1:100

Anti-NuMA antibody used in Western Blot (WB). GTX64368

GTX64368 WB Image

WB analysis of various sample lysates using GTX64368 NuMA antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NuMA antibody used in IHC (Paraffin sections) (IHC-P). GTX64368

GTX64368 IHC-P Image

IHC-P analysis of human stomach tissue using GTX64368 NuMA antibody.
Dilution : 1:100

Anti-NuMA antibody used in IHC (Paraffin sections) (IHC-P). GTX64368

GTX64368 IHC-P Image

IHC-P analysis of rat heart tissue using GTX64368 NuMA antibody.
Dilution : 1:100

REFERENCE

Application Reference

Anchel Gonz?lez-Barriga et al. Front Cell Neurosci 2021; 15 : 662035 Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399