PRODUCT
Summary
Nuclear Fast Red Counterstain
APPLICATION
Application Note
Seven Step Procedure: 1.After the end of the chromogen incubation, rinse slides with distilled water and drain.2.Introduce the Nuclear Fast Red.3.Incubate the slides for 1-2 min. at room temperature.4.Drain off the Nuclear Fast Red and wash slides several times with distilled water. 5.Dehydrate the slides by passing them through graded alcohols.6.Bring the slides to xylene or xylene substitute clearing agent.7.Mount with Clarion (Cat. No. GTX73339, GTX73340) or other organic solvent based mounting medium.
PROPERTIES
Storage
Store at room temperature.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Background
Our Nuclear Fast Red is a light red nuclear counterstain designed for counterstaining immunohistochemistry chromogens like BCIP/NBT (Cat. No. GTX73326), BCIP/INT (Cat. GTX73325), DAB Cobalt (Cat. No. GTX73331). This is an ideal counterstain to be used with the chromogens that cannot be counterstained with hematoxylin because of the little or no color contrast. Specimens counterstained with Nuclear Fast Red can be dehydrated and mounted with organic solvents-based plastic polymers such as Clarion (Cat. No. GTX73339, GTX73340) or Permount. This light red nuclear counterstain is designed for counterstaining BCIP/NBT and DAB Cobalt. Specimens counterstained with Nuclear Fast Red should be dehydrated and mounted with an organic mounting media such as Clarion (See Pg. 54). This counterstain is not compatible with aqueous mounting media. Nuclear Fast Red Counterstain is not compatible with Crystal/Mount, Gel/Mount or other aqueous mounting media.
DATA IMAGES
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GTX73305 IHC-P Image
IHC-P analysis of human tonsil using Nuclear Fast Red counterstain without antibody.
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REFERENCE
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REVIEW
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