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Nuclear Matrix Protein p84 antibody [5E10]

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunoprecipitation (IP). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

Cat No. GTX70220

Host

Mouse

Clonality

Monoclonal

Clone Name

5E10

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

Reactivity

Human, Mouse, Rat, Hamster, Monkey
Package
100 μl ($329)
PRODUCT

Summary

Nuclear Matrix Protein p84 antibody detects THO complex subunit 1, which is an 84 kDa nuclear matrix protein often referred to as just “p84”. p84 is involved in mRNA transcription and is considered to be a nuclear marker. p84 antibody is an effective reagent used to verify the purity of nuclear extracts in cellular fractionation assays.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3 - 2 μg/ml
ICC/IF 0.5 - 2 μg/ml
IHC-P Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , HeLa nucleus , NIH-3T3 , mouse brain , PC-12 , mouse Cerebellum

Product Note

Knockdown/Knockout validation was supported by references (PMID:25296641)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 15-374 of human p84 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372637

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

THO complex 1 , HPR1 , P84 , P84N5

Cellular Localization

nucleoplasm , Cytoplasm

Background

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70220

GTX70220 ICC/IF Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunoprecipitation (IP). GTX70220

GTX70220 IP Image

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (GTX70220) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (GTX70220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: HeLa nucleus
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Sample (50 μg of whole cell lysate)
A: mouse Cerebellum
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220

GTX70220 IHC-P Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220

GTX70220 IHC-P Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (GTX70220) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

REFERENCE

REVIEW

Nuclear Matrix Protein p84 antibody [5E10] Cat No. GTX70220

Rating ( Average 5 based on 12 users reviews)
Application
Western Blot(WB) ( Average 5 based on 12 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013.PMID: 23341130
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2014.PMID: 25030234
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28157211
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biomed Res Int in 2015.PMID: 25834829
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28157211
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013.PMID: 23341130
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 29045486
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 29045486
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Biomed Res Int in 2015.PMID: 25722974
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2015.PMID: 25927232
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal BMC Biochem in 2007.PMID: 17880698
Date : Anonymous submitted on 06-Feb-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : TBST with 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 329