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Nuclear Matrix Protein p84 antibody [C1C3]

KO/KD
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Western Blot (WB). GTX102919
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Western Blot (WB). GTX102919
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in ChIP assay (ChIP assay). GTX102919
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Immunoprecipitation (IP). GTX102919
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102919
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Western Blot (WB). GTX102919
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102919

Cat. No. GTX102919

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Bird
Package
100 μl ($399),
25 μl ($169)
See all Nuclear Matrix Protein p84 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat-2

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nuclear Matrix Protein p84. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630636

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

THO complex 1 , HPR1 , P84 , P84N5

Background

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 606390).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Western Blot (WB). GTX102919

GTX102919 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Western Blot (WB). GTX102919

GTX102919 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in ChIP assay (ChIP assay). GTX102919

GTX102919 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Nuclear Matrix Protein p84 antibody. The precipitated DNA was detected by PCR with primer set targeting to calreticulin.
ChIP experiment and primer designs are based on Mol Cell Biol. 2005 May;25(10):4023-33.

Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Immunoprecipitation (IP). GTX102919

GTX102919 IP Image

Nuclear Matrix Protein p84 antibody [C1C3] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 μg of Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102919

GTX102919 ICC/IF Image

Nuclear Matrix Protein p84 antibody [C1C3] detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in Western Blot (WB). GTX102919

GTX102919 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102919

GTX102919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Nuclear Matrix Protein p84 (GTX102919) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lee MT et al. Asian-Australas J Anim Sci 2019; (Epub) Effects of dietary Antrodia cinnamomea fermented product supplementation on metabolism pathways of antioxidant, inflammatory, and lipid metabolism pathways-a potential crosstalk.
Application : WB
Reactivity : Bird
Wei J et al. EMBO J 2018; 37 (22) HRD1-ERAD controls production of the hepatokine FGF21 through CREBH polyubiquitination.
Wei J et al. EMBO 2018; 37 : e98942 HRD1-ERAD controls production of the hepatokine FGF21 through CREBH polyubiquitination.
Reactivity : Mouse
Mulero MC et al. Biochemistry 2018; 57 (20) : 2943-2957 Protein Cofactors Are Essential for High-Affinity DNA Binding by the Nuclear Factor κB RelA Subunit.
Mukherjee M et al. Oncogene 2013; MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERα)-positive mammary adenocarcinomas.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169