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Nuclear Matrix Protein p84 antibody [C1C3]

KO/KD
GTX102919_40163_20180817_WB_shRNA_watermark_19011506_439.jpg
GTX102919_40163_IFA_19011506_538.jpg
GTX102919_40163_20170825_WB_R_19011506_930.jpg
GTX102919_40163_IHC_19011506_671.jpg
GTX102919_40163_ChIP_19011506_282.jpg
GTX102919_40163_IP_19011506_277.jpg
GTX102919_40163_20170825_WB_19011506_979.jpg

Cat No. GTX102919

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat-2 , THOC1 shRNA-transfected 293T

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nuclear Matrix Protein p84. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HPR1 antibody, P84 antibody, P84N5 antibody, THOC1 antibody, tho1 antibody, THO complex subunit 1 antibody, hTREX84 antibody, nuclear matrix protein p84 antibody, THO complex 1 antibody

Background

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 606390).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102919_40163_20180817_WB_shRNA_watermark_19011506_439.jpg

GTX102919 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102919_40163_IFA_19011506_538.jpg

GTX102919 ICC/IF Image

Nuclear Matrix Protein p84 antibody [C1C3] detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102919_40163_20170825_WB_R_19011506_930.jpg

GTX102919 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102919_40163_IHC_19011506_671.jpg

GTX102919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Nuclear Matrix Protein p84 (GTX102919) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102919_40163_ChIP_19011506_282.jpg

GTX102919 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Nuclear Matrix Protein p84 antibody. The precipitated DNA was detected by PCR with primer set targeting to calreticulin.
ChIP experiment and primer designs are based on Mol Cell Biol. 2005 May;25(10):4023-33.

GTX102919_40163_IP_19011506_277.jpg

GTX102919 IP Image

Nuclear Matrix Protein p84 antibody [C1C3] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 μg of Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX102919_40163_20170825_WB_19011506_979.jpg

GTX102919 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169