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Nucleobindin 1 antibody [HL2532]

Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901

Cat. No. GTX638901

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2532

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Nucleobindin 1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleobindin 1 , CALNUC , NUC

Cellular Localization

Cytoplasm,Secreted

Background

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901

GTX638901 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nucleobindin 1 antibody [HL2532] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638901

GTX638901 ICC/IF Image

Nucleobindin 1 antibody [HL2532] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901

GTX638901 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901

GTX638901 IHC-P Image

Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901

GTX638901 IHC-P Image

Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901

GTX638901 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations