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Nucleobindin 1 antibody [N3C3]

Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593

Cat No. GTX114593

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nucleobindin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619335

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleobindin 1 , CALNUC , NUC

Cellular Localization

Golgi apparatus , cis-Golgi network membrane; Peripheral membrane protein; Lumenal side , Cytoplasm

Background

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq]

Database

DATA IMAGES

Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593

GTX114593 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Nucleobindin 1(GTX114593) antibody at 1:200 dilution.

Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593

GTX114593 IHC-P Image

Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593

GTX114593 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX114593 diluted at 1:1000

Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593

GTX114593 ICC/IF Image

Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody [N3C3] (GTX114593) diluted at 1:500.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593

GTX114593 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX114593 diluted at 1:1000

Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593

GTX114593 IHC-P Image

Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Nucleobindin 1 antibody [N3C3] Cat No. GTX114593

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Dec-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA, 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169