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Nucleobindin 1 antibody [N3C3]

Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593

Cat. No. GTX114593

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Mouse brain , HeLa

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nucleobindin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleobindin 1 , CALNUC , NUC

Cellular Localization

Golgi apparatus , cis-Golgi network membrane; Peripheral membrane protein; Lumenal side , Cytoplasm

Background

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593

GTX114593 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2530] (GTX114593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593

GTX114593 IHC-P Image

Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593

GTX114593 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Nucleobindin 1(GTX114593) antibody at 1:200 dilution.

Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114593

GTX114593 IHC-P Image

Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593

GTX114593 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX114593 diluted at 1:1000

Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593

GTX114593 ICC/IF Image

Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody [N3C3] (GTX114593) diluted at 1:500.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Nucleobindin 1 antibody [N3C3] Cat. No. GTX114593

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Dec-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA, 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169