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Nucleolin antibody [364-5]

Anti-Nucleolin antibody [364-5] used in IHC (Paraffin sections) (IHC-P). GTX34916
Anti-Nucleolin antibody [364-5] used in Flow cytometry (FACS). GTX34916
Anti-Nucleolin antibody [364-5] used in IHC (Paraffin sections) (IHC-P). GTX34916
Anti-Nucleolin antibody [364-5] used in IHC (Paraffin sections) (IHC-P). GTX34916

Cat No. GTX34916

Host Mouse
Clonality Monoclonal
Clone Name 364-5
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.ĀThis MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Lysate of SU-DHL-1 Nuclei

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleolin,C23,Nsr1,Ncl

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Nucleolin antibody [364-5] used in IHC (Paraffin sections) (IHC-P). GTX34916

GTX34916 IHC-P Image

Formalin-fixed paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).

Anti-Nucleolin antibody [364-5] used in Flow cytometry (FACS). GTX34916

GTX34916 FACS Image

Flow Cytometry of Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).

Anti-Nucleolin antibody [364-5] used in IHC (Paraffin sections) (IHC-P). GTX34916

GTX34916 IHC-P Image

Formalin-fixed paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (364-5).

Anti-Nucleolin antibody [364-5] used in IHC (Paraffin sections) (IHC-P). GTX34916

GTX34916 IHC-P Image

Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 Conjugate of Nucleolin Monoclonal Antibody (364-5).