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Nucleolin antibody [4E2]

Anti-Nucleolin antibody [4E2] used in Western Blot (WB). GTX13541
Anti-Nucleolin antibody [4E2] used in Immunohistochemistry (IHC). GTX13541

Cat. No. GTX13541

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E2

Isotype

 IgG1

Application

 WB, ICC/IF, ChIP assay, IHC

Reactivity

 Human, Bovine
Package
50 μg ($399)
See all Nucleolin products

APPLICATION

Application Note

ICC: Use at a concentration of 1 - 10 μg/ml. IHC: Use at a concentration of 1 - 10 μg/ml. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 100 kDa. This antibody allows in situ detection of nucleolar protein in Hep-II cell and paraffin embedded tissue section. Paraffin embedded sections should be processed under microwave treatment. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

77 kDa. ( Note )

Positive Control

Raji cell extract

PROPERTIES

Form

Liquid

Buffer

50% Glycerol, PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human nucleolin protein from Raji cell extract.

Purification

Protein A purified
This antibody was affinity purified.

Conjugation

Unconjugated

RRID

AB_372550

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolin , C23 , Nsr1

Cellular Localization

Cytoplasm , Nucleolus

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Nucleolin antibody [4E2] used in Western Blot (WB). GTX13541

GTX13541 WB Image

Western Blot analysis using Nucleolin [4E2] antibody (GTX13541) on HL60 (1) and ZR-75 (2) cell extracts

Anti-Nucleolin antibody [4E2] used in Immunohistochemistry (IHC). GTX13541

GTX13541 IHC Image

Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).

REFERENCE

Application Reference

Villanueva E et al. Nat Protoc 2020; 15 (8) : 2568-2588 Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS).
Application : WB
Reactivity : Human
Alexandrova J et al. Biochem J 2020; 477 (4) : 773-786 Full-length NF-κB repressing factor contains an XRN2 binding domain.
Application : WB
Reactivity : Human
Lai WY et al. Biomaterials 2014; 35 (9) : 2905-14 Synergistic inhibition of lung cancer cell invasion, tumor growth and angiogenesis using aptamer-siRNA chimeras.
Application : WB
Reactivity : Human
Wei PC et al. Nucleic Acids Res 2013; 41 (3) : 1533-43 A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress.
Application : ChIP assay
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399