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Nucleolin antibody, C-term

Anti-Nucleolin antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47363
Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
Anti-Nucleolin antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47363

Cat. No. GTX47363

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Nucleolin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ICC/IF Assay dependent
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Daudi , NT-2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human NCL

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolin , C23 , Nsr1

Cellular Localization

Cytoplasm,Nucleolus

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Nucleolin antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47363

GTX47363 IHC-P Image

IHC-P analysis of human kidney tissue using GTX47363 NCL antibody at 4.0-8.0μg/ml.

Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363

GTX47363 WB Image

WB analysis of NT-2 cells using GTX47363 NCL antibody at 2μg/ml.

Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363

GTX47363 WB Image

WB analysis of Daudi cells using GTX47363 NCL antibody at 1.25μg/ml.
Lane A: marker
Lane B: Daudi cells

Anti-Nucleolin antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47363

GTX47363 ICC/IF Image

ICC/IF analysis of NT-2 cells using GTX47363 NCL antibody at 1:500.
Red: NCL
Blue:DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399