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Nucleolin antibody [MSVA-623R] HistoMAX™

HistoMAX
Anti-Nucleolin antibody [MSVA-623R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04456
Anti-Nucleolin antibody [MSVA-623R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04456
Anti-Nucleolin antibody [MSVA-623R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04456

Cat. No. GTX04456

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-623R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($679),
100 μl ($299)
See all Nucleolin products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible – at least – in the superficial epithelial cell layer.

Negative Control

Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human NCL protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolin , C23 , Nsr1

Cellular Localization

Cytoplasm , Nucleolus

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Nucleolin antibody [MSVA-623R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04456

GTX04456 IHC-P Image

IHC-P analysis of human uterus endometrium tissue section using GTX04456 Nucleolin antibody [MSVA-623R] HistoMAX™.
In this sample of endometrium nucleolin staining is particularly prominent in the nucleoli of epithelial and stromal cells.

Anti-Nucleolin antibody [MSVA-623R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04456

GTX04456 IHC-P Image

IHC-P analysis of human fallopian tube mucosa tissue section using GTX04456 Nucleolin antibody [MSVA-623R] HistoMAX™.
The entire nuclei of epithelial cells are nucleolin positive but the immunostaining is most distinct in the nucleolus.

Anti-Nucleolin antibody [MSVA-623R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04456

GTX04456 IHC-P Image

IHC-P analysis of human esophageal squamous cell carcinoma tissue section using GTX04456 Nucleolin antibody [MSVA-623R] HistoMAX™.
Distinct nucleolar immunostaining in the nucleoli of an esophageal squamous cell carcinoma.

REFERENCE

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REVIEW

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Package List Price ($)
$ 679
$ 299