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Nucleolin antibody, N-term

Anti-Nucleolin antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47364
Anti-Nucleolin antibody, N-term used in Western Blot (WB). GTX47364
Anti-Nucleolin antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47364

Cat No. GTX47364

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human NCL

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleolin , C23 , Nsr1

Cellular Localization

Cytoplasm,Nucleolus

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Nucleolin antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47364

GTX47364 IHC-P Image

IHC-P analysis of human kidney tissue using GTX47364 NCL antibody at 4.0-8.0μg/ml.

Anti-Nucleolin antibody, N-term used in Western Blot (WB). GTX47364

GTX47364 WB Image

WB analysis of Jurkat cells using GTX47364 NCL antibody at 0.2-1μg/ml.

Anti-Nucleolin antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47364

GTX47364 IHC-P Image

IHC-P analysis of human lung tissue using GTX47364 NCL antibody at 4.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf