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Nucleolin antibody [NCL/902]

GTX34914_20170712_IHC-P_18121410_449.jpg
GTX34914_20170712_IHC-P_2_18121410_364.jpg
GTX34914_20170712_WB_18121410_509.jpg
GTX34914_20170712_IHC-P_1_18121410_873.jpg

Cat No. GTX34914

Host Mouse
Clonality Monoclonal
Clone Name NCL/902
Isotype IgG1
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

WB:0.5-1ug/ml, IHC-P:0.5-1.0ug/ml for 30 minutes at RT.

Calculated MW

77 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.ĀThis MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human NCL protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleolin,C23,Nsr1,Ncl

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34914_20170712_IHC-P_18121410_449.jpg

GTX34914 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (NCL/902).

GTX34914_20170712_IHC-P_2_18121410_364.jpg

GTX34914 IHC-P Image

Formalin-fixed, paraffin-embedded human Uterus stained with Nucleolin Monoclonal Antibody (NCL/902).

GTX34914_20170712_WB_18121410_509.jpg

GTX34914 WB Image

Western Blot of A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)

GTX34914_20170712_IHC-P_1_18121410_873.jpg

GTX34914 IHC-P Image

Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (NCL/902).