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Nucleolin antibody

Anti-Nucleolin antibody used in Western Blot (WB). GTX16940
Anti-Nucleolin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16940
Anti-Nucleolin antibody used in Western Blot (WB). GTX16940
Anti-Nucleolin antibody used in IHC (Paraffin sections) (IHC-P). GTX16940

Cat. No. GTX16940

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ChIP assay, EM, IM

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all Nucleolin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:4000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
ChIP assay Assay dependent
EM 1:10 - 1:500
IM Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A fusion protein containing amino acids 284-709 of human Nucleolin. [UniProt# P19338]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolin , C23 , Nsr1

Cellular Localization

Cytoplasm,Nucleolus

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Nucleolin antibody used in Western Blot (WB). GTX16940

GTX16940 WB Image

WB analysis of HeLa cell lysate using GTX16940 Nucleolin antibody.

Anti-Nucleolin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16940

GTX16940 ICC/IF Image

ICC/IF analysis of murine pre-B cells using GTX16940 Nucleolin antibody.

Anti-Nucleolin antibody used in Western Blot (WB). GTX16940

GTX16940 WB Image

WB analysis of PD31 nuclear extracts using GTX16940 Nucleolin antibody.

Anti-Nucleolin antibody used in IHC (Paraffin sections) (IHC-P). GTX16940

GTX16940 IHC-P Image

IHC-P analysis of human tonsill germinal center using GTX16940 Nucleolin antibody.

REFERENCE

Application Reference

Sheng Li et al. STAR Protoc 2021; 2 (2) : 100601 In?vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions.
Application : WB
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419