GeneTex
United States (US)

Nucleolin antibody

GTX16940_1095_WB_18121410_220.jpg
GTX16940_298_IHC-P_18121410_882.jpg
GTX16940_139_ICC-IF_18121410_509.jpg
GTX16940_1096_WB_18121410_921.jpg

Cat No. GTX16940

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ChIP assay, EM, IM
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:4000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
ChIP assay Assay dependent
EM 1:10 - 1:500
IM Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein containing amino acids 284-709 of human Nucleolin. [UniProt# P19338]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleolin,C23,Nsr1,Ncl

Cellular Localization

Nucleus,nucleolus

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX16940_1095_WB_18121410_220.jpg

GTX16940 WB Image

WB analysis of HeLa cell lysate using GTX16940 Nucleolin antibody.

GTX16940_298_IHC-P_18121410_882.jpg

GTX16940 IHC-P Image

IHC-P analysis of human tonsill germinal center using GTX16940 Nucleolin antibody.

GTX16940_139_ICC-IF_18121410_509.jpg

GTX16940 ICC/IF Image

ICC/IF analysis of murine pre-B cells using GTX16940 Nucleolin antibody.

GTX16940_1096_WB_18121410_921.jpg

GTX16940 WB Image

WB analysis of PD31 nuclear extracts using GTX16940 Nucleolin antibody.