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Nucleophosmin antibody [5E3]

Anti-Nucleophosmin antibody [5E3] used in Flow cytometry (FACS). GTX57555
Anti-Nucleophosmin antibody [5E3] used in Western Blot (WB). GTX57555

Cat. No. GTX57555

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5E3

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($399)
See all Nucleophosmin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 5E3 is derived from hybridization of mouse SP2/2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NPM protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleophosmin 1 , B23 , NPM , NPM1 , nucleophosmin , Nucleophosmin

Cellular Localization

Nucleus,Nucleolus,Nucleoplasm,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome

Background

The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Nucleophosmin antibody [5E3] used in Flow cytometry (FACS). GTX57555

GTX57555 FACS Image

FACS analysis of HeLa cells using GTX57555 Nucleophosmin antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-Nucleophosmin antibody [5E3] used in Western Blot (WB). GTX57555

GTX57555 WB Image

WB analysis of various samples using GTX57555 Nucleophosmin antibody.
Samples : HeLa and HepG2 whole cell lysate
Loading : 5 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399