APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:200 |
| 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT23F1 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NPM3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleophosmin 1 , B23 , NPM , NPM1 , nucleophosmin , Nucleophosmin
Cellular Localization
Nucleus,Nucleolus,Nucleoplasm,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome
Background
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Nucleophosmin antibody [AT23F1] used in Flow cytometry (FACS). GTX57613](/templates/default/js/lightbox/images/loading.gif "Anti-Nucleophosmin antibody [AT23F1] used in Flow cytometry (FACS). GTX57613")
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GTX57613 FACS Image
FACS analysis of WiDr cells using GTX57613 Nucleophosmin antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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GTX57613 ICC/IF Image
ICC/IF analysis of WiDr cells using GTX57613 Nucleophosmin antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:200
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GTX57613 WB Image
WB analysis of various samples using GTX57613 Nucleophosmin antibody.
Lane 1 : Recombinant Human NPM1 protein (50 ng)
Lane 2 : Jurkat whole cell lysate (40 μg)
Lane 3 : 293T whole cell lysate (40 μg)
Lane 4 : HeLa whole cell lysate (40 μg)
Lane 5 : HepG2 whole cell lysate (40 μg)
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REFERENCE
There are currently no references for Nucleophosmin antibody [AT23F1] (GTX57613). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Nucleophosmin antibody [AT23F1] (GTX57613). Be the first to share your experience with this product.
![Anti-Nucleophosmin antibody [AT23F1] used in Flow cytometry (FACS). GTX57613](/upload/website/prouct_img/small/GTX57613/GTX57613_20200204_FC_w_23061123_741.webp "Anti-Nucleophosmin antibody [AT23F1] used in Flow cytometry (FACS). GTX57613")
![Anti-Nucleophosmin antibody [AT23F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57613](/upload/website/prouct_img/small/GTX57613/GTX57613_20200204_IF_w_23061123_568.webp "Anti-Nucleophosmin antibody [AT23F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57613")
![Anti-Nucleophosmin antibody [AT23F1] used in Western Blot (WB). GTX57613](/upload/website/prouct_img/small/GTX57613/GTX57613_20200204_WB_w_23061123_884.webp "Anti-Nucleophosmin antibody [AT23F1] used in Western Blot (WB). GTX57613")
![Anti-Nucleophosmin antibody [AT23F1] used in Flow cytometry (FACS). GTX57613](/upload/website/prouct_img/normal/GTX57613/GTX57613_20200204_FC_w_23061123_741.webp "Anti-Nucleophosmin antibody [AT23F1] used in Flow cytometry (FACS). GTX57613")
![Anti-Nucleophosmin antibody [AT23F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57613](/upload/website/prouct_img/normal/GTX57613/GTX57613_20200204_IF_w_23061123_568.webp "Anti-Nucleophosmin antibody [AT23F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57613")
![Anti-Nucleophosmin antibody [AT23F1] used in Western Blot (WB). GTX57613](/upload/website/prouct_img/normal/GTX57613/GTX57613_20200204_WB_w_23061123_884.webp "Anti-Nucleophosmin antibody [AT23F1] used in Western Blot (WB). GTX57613")