APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:10-1:50 |
| 1:10-1:50 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 7-33 amino acids from the N-terminal region of human NPM1.
Purification
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleophosmin 1 , B23 , NPM
Cellular Localization
nucleoplasm,Nucleolus
Background
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX81996 IHC-P Image
IHC-P analysis of human lymph tissue using GTX81996 Nucleophosmin antibody, N-term.
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GTX81996 WB Image
WB analysis of HeLa cell lysate (35ug/lane) using GTX81996 Nucleophosmin antibody, N-term.
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GTX81996 FACS Image
FACS analysis of HeLa cells using GTX81996 Nucleophosmin antibody, N-term.
Right histogram : HeLa cells
Left histogram : negative control
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GTX81996 ICC/IF Image
ICC/IF analysis of A549 cells using GTX81996 Nucleophosmin antibody, N-term.
Green : Nucleophosmin
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25
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REFERENCE
There are currently no references for Nucleophosmin antibody, N-term (GTX81996). Be the first to share your publications with this product.
REVIEW
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