GeneTex
United States (US)

Nucleophosmin (phospho Thr234) antibody

GTX32229_20190131_WB_19013101_215.jpg

Cat No. GTX32229

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Nucleophosmin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleophosmin 1,B23,Npm,Npm1

Cellular Localization

Centrosome,Cytoplasm,Cytoskeleton,Microtubule organizing center,Nucleolus,Nucleoplasm,Nucleus

Background

The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
GTX32229_20190131_WB_19013101_215.jpg

GTX32229 WB Image

WB analysis of colchicine-treated MCF7 (A), LNCaP (B), HeLa (C), colchicine-treated Raw264.7 (D), colchicine-treated PC12 (E) whole cell lysates using GTX32229 Nucleophosmin (phospho Thr234) antibody.

Package List Price ($)
$ 319