APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888154
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleoporin 62 , IBSN , SNDI , p62
Cellular Localization
Nucleus , nuclear pore complex
Background
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Nucleoporin p62 antibody [GT252] used in Western Blot (WB). GTX629763](/templates/default/js/lightbox/images/loading.gif "Anti-Nucleoporin p62 antibody [GT252] used in Western Blot (WB). GTX629763")
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GTX629763 WB Image
Nucleoporin p62 antibody [GT252] detects Nucleoporin p62 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Nucleoporin p62 antibody [GT252] (GTX629763) dilution: 1:1000
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GTX629763 ICC/IF Image
Nucleoporin p62 antibody [GT252] detects Nucleoporin p62 protein at nuclear envelope by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody [GT252] (GTX629763) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
There are currently no references for Nucleoporin p62 antibody [GT252] (GTX629763). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Nucleoporin p62 antibody [GT252] (GTX629763). Be the first to share your experience with this product.
![Anti-Nucleoporin p62 antibody [GT252] used in Western Blot (WB). GTX629763](/upload/website/prouct_img/small/GTX629763/GTX629763_41456_WB_w_23061202_369.webp "Anti-Nucleoporin p62 antibody [GT252] used in Western Blot (WB). GTX629763")
![Anti-Nucleoporin p62 antibody [GT252] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629763](/upload/website/prouct_img/small/GTX629763/GTX629763_41456_IFA_w_23061202_611.webp "Anti-Nucleoporin p62 antibody [GT252] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629763")
![Anti-Nucleoporin p62 antibody [GT252] used in Western Blot (WB). GTX629763](/upload/website/prouct_img/normal/GTX629763/GTX629763_41456_WB_w_23061202_369.webp "Anti-Nucleoporin p62 antibody [GT252] used in Western Blot (WB). GTX629763")
![Anti-Nucleoporin p62 antibody [GT252] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629763](/upload/website/prouct_img/normal/GTX629763/GTX629763_41456_IFA_w_23061202_611.webp "Anti-Nucleoporin p62 antibody [GT252] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629763")