GeneTex
United States (US)

Nucleoporin p62 antibody

GTX102359_39820_20141205_IHC_M_19011506_298.jpg
GTX102359_39820_WB_R_19011506_641.jpg
GTX102359_WB_19011506_624.jpg
GTX102359_39820_WB_M_19011506_936.jpg

Cat No. GTX102359

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A431 , H1299 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp547L134 antibody, FLJ20822 antibody, FLJ43869 antibody, IBSN antibody, MGC841 antibody, SNDI antibody, p62 antibody, NUP62 antibody, 62 kDa nucleoporin antibody, nucleoporin Nup62 antibody, nuclear pore glycoprotein p62 antibody, nucleoporin 62kDa antibody

Cellular Localization

Nucleus , nuclear pore complex

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102359_39820_20141205_IHC_M_19011506_298.jpg

GTX102359 IHC-P Image

Nucleoporin p62 antibody detects Nucleoporin p62 protein at nuclear envelope on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Nucleoporin p62 antibody (GTX102359) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102359_39820_WB_R_19011506_641.jpg

GTX102359 WB Image

Nucleoporin p62 antibody detects NUP62 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
Nucleoporin p62 antibody (GTX102359) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102359_WB_19011506_624.jpg

GTX102359 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
nucleoporin p62 antibody
GTX102359 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102359_39820_WB_M_19011506_936.jpg

GTX102359 WB Image

Nucleoporin p62 antibody detects NUP62 protein by western blot analysis.
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
10% SDS-PAGE
Nucleoporin p62 antibody (GTX102359) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169