APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , A375 , NIH-3T3 , JC , BCL-1 , C2C12 , PC-12
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.86 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951043
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleoporin 62 , IBSN , SNDI , p62
Cellular Localization
Nucleus , nuclear pore complex
Background
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX107920 WB Image
Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX107920 diluted at 1:5000
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GTX107920 WB Image
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7.5% SDS PAGE GTX107920 diluted at 1:5000
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GTX107920 WB Image
Sample (30 ug of whole cell lysate) A: A431 B: A375 7.5% SDS PAGE GTX107920 diluted at 1:10000
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REFERENCE
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REVIEW
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