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Nucleoporin p62 antibody

Anti-Nucleoporin p62 antibody used in Western Blot (WB). GTX107973
Anti-Nucleoporin p62 antibody used in Immunoprecipitation (IP). GTX107973
Anti-Nucleoporin p62 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107973
Anti-Nucleoporin p62 antibody used in IHC (Paraffin sections) (IHC-P). GTX107973
Anti-Nucleoporin p62 antibody used in IHC (Paraffin sections) (IHC-P). GTX107973
Anti-Nucleoporin p62 antibody used in Western Blot (WB). GTX107973
Anti-Nucleoporin p62 antibody used in Western Blot (WB). GTX107973

Cat. No. GTX107973

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Nucleoporin p62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , C2C12 , PC-12 , HepG2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951041

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleoporin 62 , IBSN , SNDI , p62

Cellular Localization

Nucleus , nuclear pore complex

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Nucleoporin p62 antibody used in Western Blot (WB). GTX107973

GTX107973 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
7.5% SDS PAGE
GTX107973 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nucleoporin p62 antibody used in Immunoprecipitation (IP). GTX107973

GTX107973 IP Image

Nucleoporin p62 antibody immunoprecipitates NUP62 protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of NUP62 protein by 4 μg Nucleoporin p62 antibody (GTX107973)
7.5 % SDS-PAGE
The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody (GTX107973) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Nucleoporin p62 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107973

GTX107973 ICC/IF Image

Nucleoporin p62 antibody detects Nucleoporin p62 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody (GTX107973) diluted at 1:750.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Nucleoporin p62 antibody used in IHC (Paraffin sections) (IHC-P). GTX107973

GTX107973 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using nucleoporin p62 (GTX107973) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nucleoporin p62 antibody used in IHC (Paraffin sections) (IHC-P). GTX107973

GTX107973 IHC-P Image

Nucleoporin p62 antibody detects Nucleoporin p62 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Nucleoporin p62 stained by Nucleoporin p62 antibody (GTX107973) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nucleoporin p62 antibody used in Western Blot (WB). GTX107973

GTX107973 WB Image

Nucleoporin p62 antibody detects Nucleoporin p62 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody (GTX107973) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nucleoporin p62 antibody used in Western Blot (WB). GTX107973

GTX107973 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX107973 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Li-Li Wen et al. PLoS One 2021; 16 (1) : e0245442 Perfluorooctane sulfonate induces autophagy-associated apoptosis through oxidative stress and the activation of extracellular signal-regulated kinases in renal tubular cells.
Application : WB
Reactivity : Rat
Rodriguez-Bravo V et al. Cell 2018; (Epub) Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import.
Application : WB
Reactivity : Human
Gu Y et al. Biochem Pharmacol 2018; 152 : 21-33 Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
Application : WB
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169