GeneTex
United States (US)

Nucleoporin p62 antibody

GTX107973_39988_WB_R_19011507_805.jpg
GTX107973_41059_WB_19011507_798.jpg
GTX107973_39988_IP_19011507_843.jpg
GTX107973_39988_WB_M_19011507_972.jpg
GTX107973_39988_20160224_IFA_19011507_647.jpg
GTX107973_IHC_19011507_238.jpg

Cat No. GTX107973

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3 , JC , BCL-1 , C2C12 , PC-12

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleoporin 62,Ibsn,Sndi,P62,Nup62

Cellular Localization

Nucleus , nuclear pore complex

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107973_39988_WB_R_19011507_805.jpg

GTX107973 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX107973 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107973_41059_WB_19011507_798.jpg

GTX107973 WB Image

Nucleoporin p62 antibody detects Nucleoporin p62 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody (GTX107973) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107973_39988_IP_19011507_843.jpg

GTX107973 IP Image

Nucleoporin p62 antibody immunoprecipitates NUP62 protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of NUP62 protein by 4 μg Nucleoporin p62 antibody (GTX107973)
7.5 % SDS-PAGE
The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody (GTX107973) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX107973_39988_WB_M_19011507_972.jpg

GTX107973 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
7.5% SDS PAGE
GTX107973 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107973_39988_20160224_IFA_19011507_647.jpg

GTX107973 ICC/IF Image

Nucleoporin p62 antibody detects Nucleoporin p62 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody (GTX107973) diluted at 1:750.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX107973_IHC_19011507_238.jpg

GTX107973 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using nucleoporin p62 (GTX107973) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169