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Nucleostemin antibody

Anti-Nucleostemin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54163
Anti-Nucleostemin antibody used in Western Blot (WB). GTX54163
Anti-Nucleostemin antibody used in Immunoprecipitation (IP). GTX54163

Cat. No. GTX54163

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all Nucleostemin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein nucleolar 3 , C77032 , E2IG3 , NNP47 , NS

Cellular Localization

Nucleus,Nucleolus

Background

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Nucleostemin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54163

GTX54163 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX54163 Nucleostemin antibody.
Blue : DAPI

Anti-Nucleostemin antibody used in Western Blot (WB). GTX54163

GTX54163 WB Image

WB analysis of 293T cell lysate using GTX54163 Nucleostemin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Nucleostemin antibody used in Immunoprecipitation (IP). GTX54163

GTX54163 IP Image

IP analysis of 293T cell lysate using GTX54163 Nucleostemin antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399