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Nup153 antibody, N-term

Anti-Nup153 antibody, N-term used in Flow cytometry (FACS). GTX80552
Anti-Nup153 antibody, N-term used in Western Blot (WB). GTX80552

Cat. No. GTX80552

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Nup153 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of human NUP153.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleoporin 153 , HNUP153 , N153

Cellular Localization

Nucleus

Background

Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Nup153 antibody, N-term used in Flow cytometry (FACS). GTX80552

GTX80552 FACS Image

FACS analysis of 293 cells using GTX80552 Nup153 antibody, N-term.
Top histogram : negative control
Bottom histogram : 293 cells

Anti-Nup153 antibody, N-term used in Western Blot (WB). GTX80552

GTX80552 WB Image

WB analysis of HeLa cell lysate (35ug/lane) using GTX80552 Nup153 antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399