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Nup153 antibody [R4C8]

Anti-Nup153 antibody [R4C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00714
Anti-Nup153 antibody [R4C8] used in Western Blot (WB). GTX00714
Anti-Nup153 antibody [R4C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00714
Anti-Nup153 antibody [R4C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00714

Cat No. GTX00714

Host

Rat

Clonality

Monoclonal

Clone Name

R4C8

Isotype

IgG2a

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
200 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant GST-fused rat Nup153 (610-1191aa, Uniprot#P49791)
Epitope: 610-1191 aa (Zn finger and FG repeats domain)

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleoporin 153 , HNUP153 , N153

Cellular Localization

Nucleus

Background

Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Nup153 antibody [R4C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00714

GTX00714 ICC/IF Image

ICC/IF analysis of rat neuron cells using GTX00714 Nup153 antibody [R4C8].
The dots are Nup153.

Anti-Nup153 antibody [R4C8] used in Western Blot (WB). GTX00714

GTX00714 WB Image

WB analysis of various samples using GTX00714 Nup153 antibody [R4C8].
Lane 1 : HeLa whole cell lysate
Lane 2 : NIH3T3 whole cell lysate

Anti-Nup153 antibody [R4C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00714

GTX00714 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00714 Nup153 antibody [R4C8].

Anti-Nup153 antibody [R4C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00714

GTX00714 ICC/IF Image

ICC/IF analysis of MEF cells from E14.5 mouse embryo using GTX00714 Nup153 antibody [R4C8].
Dilution : 1:200
Fixation : 4% PFA at room temperature for 10 min
Permeabilization : Ice-Cold Methanol on ice for 10 min

REFERENCE

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REVIEW

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